Nicole Pratt is the only Australian woman left in the singles draw at Wimbledon as Samantha Stosur and Alicia Molik bowed out in the second round.

Pratt beat compatriot Casey Dellacqua, the 22-year-old qualifier from Perth, in the first round 6-3 6-4 to set up her meeting with Czech No.14 seed Nicole Vaidisova on Wednesday night (AEST).

"It's going to be a very tough match, she's a big, hard-hitting girl, big serve," Pratt said.

"I will have to serve well and I will have to try and find a way to make some returns deep down the middle so she can't get a good crack at that next ball.

"It will be pretty much a matter of me holding serve and finding a way to make some returns."

Pratt is still managing an arm injury she picked up just before last month's French Open, where she bowed out in the second round.

"The doctors told me in Paris that I'd be playing with pain for at least three to four weeks so it's just a matter of managing it and trying to stay on top of it so I can still play," she said.

"That's the reason why I couldn't spend so much time out on court - it was a progression every day, 15 minutes, 20 minutes, half an hour, 45 and I got up to an hour just a couple of days ago.

"I knew if I could get to an hour that's enough to get me through a match here at Wimbledon ... the doctor tells me, win in two sets."

Alicia Molik gave No.7 seed Serena Williams an early scare, but bowed out in a 7-6 (7-4) 6-3 second-round loss.

The Australian led 4-1 with Williams serving at 0-30 before the American launched her comeback.

"I had a number of chances today to first of all steal the first set and then to come back in that second set," Molik said.

"I see it more as a disappointment because it was a huge opportunity for myself to beat someone like Serena."

Australian No.27 seed Samantha Stosur also exited in the second round when she was upset by unseeded Venezuelan Milagros Sequera 6-2 5-7 6-4.

"I felt fine, it was just a bad game and a bad start and just automatically behind the eight ball again and you can't afford to do that and you're not going to always get out of it," Stosur said.

"Milly kept the pressure on me today."

All the Australian men in the singles draw will be in second-round action on Thursday night (AEST).

No.16 seed Lleyton Hewitt takes on Italy's Simone Bolelli, while Chris Guccione faces Russian sixth seed Nikolay Davydenko and qualifier Wayne Arthurs meets No.11 seed Tommy Robredo of Spain.

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