England flyhalf Jonny Wilkinson will miss England's opening World Cup Pool A match against the US on Saturday after twisting an ankle in training on Tuesday.

Wilkinson, who kicked the winning drop kick against Australia in the 2003 World Cup final, will be replaced by Olly Barkley.

"He has gone for a scan and I will know more in 24 hours," coach Brian Ashton told reporters.

England - 15-Mark Cueto, 14-Josh Lewsey, 13-Jamie Noon, 12-Mike Catt, 11-Jason Robinson, 10-Olly Barkley, 9-Shaun Perry, 8-Lawrence Dallaglio, 7-Tom Rees, 6-Joe Worsley, 5-Ben Kay, 4-Simon Shaw, 3-Phil Vickery, 2-Mark Regan, 1-Andrew Sheridan.

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