Two Parramatta rugby league juniors have tested positive to a banned substance the NRL club says was given to them in an "honest mistake".

The Sun-Herald reports the players, Tom Humble and Brendan Oake, are serving a provisional suspension for testing positive to a banned substance, believed to be the stimulant dimethylpentylamine (methylhexaneamine).

Both have denied knowingly taking any illegal agents and the charges will be defended at a NSWRL tribunal hearing, tentatively set down for May 19.

Both were drug-tested after playing for Wentworthville in the final round of the NSW Cup last year, the newspaper said.

It is alleged the players were given the substance by Wentworthville staff in the form of a supplement commercially known as Jack3d. The Magpies staffers are no longer employed by the club.

Parramatta chief executive Paul Osborne told the newspaper Humble and Oake had not been sighted this year for the Eels or Magpies because of the suspension.

"It was an honest mistake; they were provided with it and there was no attempt on their part to take [a banned drug]," Osborne said.

"We'd love to have them back on the paddock soon. We'll be defending it vigorously."

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